DNA Extraction and Quantitation with qPCR

Class Code: 
R104
Subject Area: 
R - Biology / DNA Program
Class Location: 
California Criminalistics Institute/Richmond or Los Angeles
Class Description: 

This 5-day course (40-hour) provides students with a basic understanding of the theory and practice of DNA extraction as well as in-depth information on DNA quantification by real time qPCR. The course focuses on current DNA extraction methods and qPCR data analysis and interpretation. The course has an emphasis on laboratory exercises. Topics covered include: 1) relevant aspects of DNA chemistry, 2) evidence handling prior to DNA analysis, 3) theoretical basis of DNA extraction methods including organic extraction, silica matrix-based methods, silica-coated magnetic beads, and PrepFiler, 4) differential extraction of sexual assault evidence, 5) quantitation of DNA by RealTime PCR (qPCR); and 6) practical experience with extraction and quantitation of single-source bloodstains, buccal swabs, and mock sexual assault samples, and extraction blanks. Each class is limited to 12 students.

Instructor: 
Various
Teaching Methods: 
Classroom lectures, group discussions, and laboratory exercises
Prerequisites: 
SWGDAM required courses or consent of class coordinator
Preparation: 
Pre-course reading material may be assigned.
Tuition: 
A tuition fee of $600 will be required for private sector or out-of-state applicants. Make checks payable to the California Department of Justice.
Course Contact: 
Neda Khoshkebari (916) 464-5599, (916)464-0600
More Information: 

cci@doj.ca.gov

Schedule of Classes:

Location Date(s)
TBD TBD

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